imarktm microplate absorbance reader  (Bio-Rad)

Article Title: Radiofrequency Radiation Alters Redox Homeostasis, ATP Status, and Apoptosis-Associated Responses in Human Ovarian Epithelial Cells.
Article Snippet: .. After 15 min of incubation at room temperature, the absorbance was measured in an iMarkTM Microplate Absorbance Reader (Bio-Rad). ..

Article Title: Isolation, Identification and In Silico Evaluation of Novel Cholinesterase Inhibitors from Terminalia triptera Stapf.
Article Snippet: .. Subsequently, substrate solution (40 μL) and DTNB reagent (5,5′-dithiobis(2-nitrobenzoic acid), 0.005 M in distilled water, 40 μL) were added, and the reaction was further incubated for 15 min. Absorbance was measured at 412 nm using an iMarkTM Microplate Absorbance Reader (Bio-Rad Laboratories, Hercules, CA, USA). ..

Article Title: Radiofrequency Radiation Alters Redox Homeostasis, ATP Status, and Apoptosis-Associated Responses in Human Ovarian Epithelial Cells.
Article Snippet: .. After 15 min of incubation at room temperature, the absorbance was measured in an iMarkTM Microplate Absorbance Reader (Bio-Rad). ..

Article Title: Chilean Honey as Alternative Antibacterial: In Vitro Activity Against Multidrug-Resistant Canine Bacterial Pathogens.
Article Snippet: In brief, 10 μL of the extract was mixed with 1.5 mL of 95% ethanol (Ibi Scientific, no. 15720, Dubuque, IA, USA), 2.7 μL of 10% aluminum chloride (Sigma-Aldrich, St. Louis, MO, USA), 2.7 μL of 1 M potassium acetate (Sigma-Aldrich), and 102.7 μL of distilled water, and incubated for 40 min at room temperature. .. The samples were read at 415 nm (iMarkTM Microplate Absorbance Reader, series 11293, Bio-Rad Laboratories, Hercules, CA, USA) and quantified against a standard curve prepared with quercetin (Sigma-Aldrich, St. Louis, https://doi.org/10.3390/ani16071125 MO, USA). ..

Article Title: Chilean Honey as Alternative Antibacterial: In Vitro Activity Against Multidrug-Resistant Canine Bacterial Pathogens
Article Snippet: In brief, 10 μL of the extract was mixed with 1.5 mL of 95% ethanol (Ibi Scientific, no. 15720, Dubuque, IA, USA), 2.7 μL of 10% aluminum chloride (Sigma-Aldrich, St. Louis, MO, USA), 2.7 μL of 1 M potassium acetate (Sigma-Aldrich), and 102.7 μL of distilled water, and incubated for 40 min at room temperature. .. The samples were read at 415 nm (iMarkTM Microplate Absorbance Reader, series 11293, Bio-Rad Laboratories, Hercules, CA, USA) and quantified against a standard curve prepared with quercetin (Sigma-Aldrich, St. Louis, MO, USA). ..

Article Title: Isolation, Identification and In Silico Evaluation of Novel Cholinesterase Inhibitors from Terminalia triptera Stapf.
Article Snippet: .. Subsequently, substrate solution (40 μL) and DTNB reagent (5,5′-dithiobis(2-nitrobenzoic acid), 0.005 M in distilled water, 40 μL) were added, and the reaction was further incubated for 15 min. Absorbance was measured at 412 nm using an iMarkTM Microplate Absorbance Reader (Bio-Rad Laboratories, Hercules, CA, USA). ..

Article Title: Intranasal Administration of SARS-CoV-2 ORF8 Accessory Protein Increases Blood Pressure and Oxidative Stress in Different Tissues of Male BALB/c Mice
Article Snippet: The supernatant was separated, and protein was quantified by the Bradford method, using the Protein Assay Dye Reagent Concentrate (Bio-Rad, Hercules, CA, USA, Cat. 0469311001) with bovine serum albumin as a standard. .. The results were read using the iMarkTM Microplate Absorbance Reader (Bio-Rad, Cat. 5000006). .. The supernatants were stored −70 °C with the cOmpleteTM Protease Inhibitor Cocktail (Sigma, Cat. 18-1130), until colorimetric determination.

Article Title: Phytochemistry and Wound-Healing, Enzyme-Inhibitory, and Antifungal Activities of the Wild Forage Legume Lotus rectus L.
Article Snippet: .. All absorbance measurements were performed using an iMarkTM microplate absorbance reader (Bio-Rad Laboratories, Inc., Hercules, CA, USA). .. Phytochemical characterization was performed by ultra-high-performance liquid chromatography coupled to high-resolution tandem mass spectrometry (UHPLC-HRMS/MS) using a Dionex Ultimate 3000 RS UHPLC system (Thermo Fisher Scientific, San Jose, CA, USA) coupled to a Q-Exactive Orbitrap high-resolution mass spectrometer equipped with a heated electrospray ionization source (HESI-II).

Article Title: Intranasal Administration of SARS-CoV-2 ORF8 Accessory Protein Increases Blood Pressure and Oxidative Stress in Different Tissues of Male BALB/c Mice.
Article Snippet: Commercially available kits were used to determine total antioxidant capacity (TAC) (Invitrogen, Cat. EIAFECL2), superoxide dismutase (SOD) (Invitrogen, Cat. EIASODC) and catalase (CAT) (Invitrogen, Cat. EIACATC) activities, as well as total glutathione concentration (GSHT) (Cayman Chemical, Cat. 703002). .. The manufacturer’s instructions were strictly followed during every assay, and results were read using the iMarkTM Microplate Absorbance Reader (Bio-Rad, Cat. 5000006). ..

Article Title: Dynamic profile of malondialdehyde in renal and hepatic ischemia reperfusion injury: an explorative study of internal historical samples
Article Snippet: At 70°C, in the presence of 37% hydrochloric acid (75 μL; 258148, Sigma-Aldrich, USA), MDA reacts with 1-Methyl-2-phenylindole, producing a purple-blue colored adduct. .. Following centrifugation (10 minutes, 4°C, 15,000g), the absorbance of the supernatant was measured at 595 nm using an iMarkTM microplate absorbance reader (Bio-Rad, USA). .. MDA concentration was calculated using a standard curve generated from 1,1,3,3-tetramethoxypropane (102-52-3, Acros Organics, Belgium) as the source of MDA.